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The establishment of EVISA is funded by the EU through the Fifth Framework Programme (G7RT- CT- 2002- 05112).


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Certified reference materials for mercury in marine animal tissues


Monomethylmercury compounds are of great concern, as these highly toxic compounds are formed biotically by microorganisms in sediment and algae in sea water and/or abiotically. Once it is formed, methylmercury is bioaccumulated and biomagnified in the aquatic food chains, thus resulting in exposure levels among fish-eating populations, often at levels exceeding what is regarded as a safe level.

It is therefore of primary interest to have samples from animal species within the food chain at different trophic level.


The main issues to be controlled during the analysis of biological tissues for the speciation analysis of mercury are:
  • quantitativeness of extraction of the species from the tissue
  • absence of artificial formation of methylmercury during the analytical procedure
  • control of contamination mainly for inorganic mercury
  • control of the derivatization yield
  • avoidance of ion-exchange and adsorption problems on GC column packing materials
CRMs play a major role in the development and validation of methods and for quality control. The following certified reference materials (CRMs) are available for the speciation of mercury species in marine animal tissues:
 
Supplier
 Name
 Material
 Certificate
IRMM
 ERM-CE464
 Tuna fish muscle
 Total mercury, methylmercury
IAEA IAEA 407
 Sea fish tissue
 (Ag),Al, As, Br, Ca, Cd, Co, Cr, Cu, Fe, Hg, MeHg, K, Li, Mg, Mn, Na, Ni, Pb, Rb, Sb, Se, Sr, V, Zn
NIST
 SRM 1566 b
 Oyster tissue
 Ca, Cl, Mg, K, Na, S, Al, As, Cd, Co, Cu, Fe, Pb, Mn, Hg, MeHg, Ni, Rb, Se, Ag, Th, V, Zn
NIST SRM 1974 B
 Mussel tissue
 Hg, methylmercury, Organics
NIST SRM 2976
 Mussel tissue
 As, Cd, Cu, Fe, Pb, Se, Zn, Total Hg, MeHg
NIST SRM 2977
 Mussel tissue
 Cd, Cu, Pb, Mn, Ni, Sr, PAHs, PCBs, MeHg
NIST SRM 1946
 Freshwater fish
NRCC
 		DORM-2
 		Dogfish muscle
 		Al, As, Cd, Co, Cr, Cu, Fe, Pb, Mn, Hg, Ni, Se, Ag, Tl, Sn, Zn, MeHg
NRCC
DORM-3
Fish protein
As, Cd, Cu, Cr, Fe, Pb, Hg, Ni, Sn, Zn, MeHg
NRCC DOLT-3
 		Dogfish liver
 		As, Cd, Cu, Fe, Pb, Hg, Ni, Se, Ag, (MeHg)
NRCC
DOLT-4
Dogfish liver
As, Cd, Cu, Fe, Pb, Hg, Ni, Se, Ag, Zn, MeHg
NRCC LUTS-1
 Non-defatted lobster tissue
 As, Cd, Ca, Cr, Co, Cu, Fe, Pb, Mg, Mn, Ni, K, Se, Ag, Sr, Zn, [Hg], [MeHg]
NRCC TORT-2
 Lobster tissue
 As, Cd, Co, Cr, Cu, Fe, Pb, Mn, Hg, Mo, Ni, Se, Sr, Sn, V, Zn, MeHg

* () =information value only, []= original certification no longer valid
NB:! click on the suppliers name to reach his website, click on the material name for a link to the report, click on the certified species for a link to the certificate

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last time modified: July 22, 2008


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