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The establishment of EVISA is funded by the EU through the Fifth Framework Programme (G7RT- CT- 2002- 05112).


Supporters of EVISA includes:

Certified reference materials for mercury in marine animal tissues



It is therefore of primary interest to have samples from the animal species within the food chain at different trophic level.


The main issues to be controlled during the analysis of biological tissues for the speciation analysis of mercury are:
  • quantitativeness of extraction of the species from the tissue
  • absence of artificial formation of methylmercury during the analytical procedure
  • control of contamination mainly for inorganic mercury
  • avoidance of ion-exchange and adsorption problems on GC coilumn packing materials
  • control of the derivatization yield
CRMs play a major role in the development and validation of methods and for quality control. The following certified reference materials (CRMs) are available for the speciation of mercury species in marine animal tissues:
 
Supplier  Name           
Material
Certificate
BCR CRM 422
Cod fish muscle

BCR CRM 463
Tuna fish musle
Total mercury, methylmercury
BCR CRM 464
Tuna fish muscle
Total mercury, methylmercury
IAEA
IAEA 140 
Marine algae homogenate

IAEA IAEA 407
Sea fish tissue
(Ag),Al, As, Br, Ca, Cd, Co, Cr, Cu, Fe, Hg, MeHg, K, Li, Mg, Mn, Na, Ni, Pb, Rb, Sb, Se, Sr, V, Zn
IAEA IAEA 346
Tuna
Hg, MeHg, under preparation
NIST
SRM 1566 b
Oyster tissue
Ca, Cl, Mg, K, Na, S, Al, As, Cd, Co, Cu, Fe, Pb, Mn, Hg, MeHg, Ni, Rb, Se, Ag, Th, V, Zn
NIST SRM 1974 B
Mussel tissue
Hg, MeHg, Organics
NIST SRM 2976
Mussel tissue
As, Cd, Cu, Fe, Pb, Se, Zn, Total Hg, MeHg
NIST SRM 2977
Mussel tissue
Cd, Cu, Pb, Mn, Ni, Sr, PAHs, PCBs, MeHg
NIST SRM 1946
Freshwater fish

NRCC
DORM-2
Dogfish muscle
Al, As, Cd, Co, Cr, Cu, Fe, Pb, Mn, Hg, Ni, Se, Ag, Tl, Sn, Zn, MeHg
NRCC DOLT-3
Dogfish liver
As, Cd, Cu, Fe, Pb, Hg, Ni, Se, Ag, (MeHg)
NRCC LUTS-1
Non-defatted lobster tissue
As, Cd, Ca, Cr, Co, Cu, Fe, Pb, Mg, Mn, Ni, K, Se, Ag, Sr, Zn, [Hg], [MeHg]
NRCC TORT-2
Lobster tissue
As, Cd, Co, Cr, Cu, Fe, Pb, Mn, Hg, Mo, Ni, Se, Sr, Sn, V, Zn, MeHg

* () =information value only, []= original certification no longer valid
NB:! click on the suppliers name to reach his website, click on the material name for a link to the report, click on the certified species for a link to the certificate

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