Certified Reference Material No.11, Fish Tissue is intended primarily for use in developing and validating analytical methods for the determination of total tin, tributyltin and triphenyltin in fish tissue or in other similar matrices.
Sea bass (Leteolabrex Japonicus) was selected because sea bass and mussel (Mytilus edulis) have been routinely used in the surveys on bioaccumulation of organotins being conducted by the Environment Agency of Japan. About 150 kg of sea bass collected in early July 1988 in Tokyo Bay was used. The fish samples (body length 50-60 cm, individual weight 1-2 kg) were stored in a liquid nitrogen jar until use.
After thawing, the fish samples were dissected with a stainless-steel knife and only the fish fillets (50 kg) were used for further processing. These were cut into small pieces and a batch of about 2 kg were minced at 3,000 rpm for 3 min with a Tecator 1094 homogenizer. After repeating this mincing procedure for the remaining batches, the fish tissue homogenate was combined and freeze-dried in one lot.
The dry fish tissue (about 12 kg) was alumina ball-milled for 1 hr and then mixed in a V-blender for 2 hrs. Twenty gram samples of the homogenized fish tissue were packaged into polyethylene bags, which were subsequently vacuum-packed doubly into polyethylene laminate bags together with an oxgen absorber. The prepared NIES Fish Tissue reference material (600 samples, 20 g each) are currently stored at -20℃.
keywords: biological material , animal tissue , foodstuff , fish/shellfish , trace elements , speciation analysis , tin speciation