Applied Biosystems - 4000 QTRAP LC/MS/MS System
| Year of introduction
Hyphenated Techniques: LC-MS
Mass spectrometer: ESI-MS
Mass spectrometer: LC-MS
The 4000 Q TRAP® LC/MS/MS System is our premier hybrid triple quadrupole/linear ion trap mass spectrometer. Ideal for drug discovery and development, metabolite identification and proteomic applications including post-translationally modified proteins discovery, protein identification, and biomarker validation.
Innovation in Linear Ion Trap Technology
- Enables high-sensitivity full-scan MS, MS/MS, and MS3 with high-selectivity from true triple quadrupole precursor ion (PI) and neutral loss (NL) scans.
- Perform multiple reaction monitoring (MRM) for quantitation using high-sensitivity triple quadrupole.
- Identify, characterize, and quantitate metabolites more quickly and easily.
- Identify and sequence post-translationally modified (PTM) peptides automatically in a single LC/MS/MS run.
- Facilitates hypothesis-driven biomarker discovery and validation.
Applied Biosystems/MDS SCIEX Linear Ion Trap (LIT) technology enables unmatched quantitative and qualitative performance within a single system. Use of a quadrupole as a linear ion trap significantly enhances ion trap performance by increasing ion capacity, improving injection and trapping efficiencies, and increasing duty cycle. With the unequalled sensitivity of the API 4000™ system, the 4000 Q TRAP® system brings LIT technology together with the leading triple quadrupole for drug development.
A Complete, Flexible, Integrated System
The 4000 Q TRAP® system fits seamlessly into your lab's workflow and boosts your discovery productivity. Together with our powerful Analyst® software, this system offers a full complement of features to simplify every aspect of methods development, data acquisition and processing, including flexible control of popular LC platforms and 21 CFR Part 11 compliance. The system design enables plug-and-play sources to accommodate a wide range of applications and flow rates. Finally, you can pull it all together with advanced application software, including Metabolite ID, BioAnalyst™, Pro ID, Pro BLAST, Pro ICAT, Fragment Interpretation, Library Searching, and Automaton, so you can obtain more useful information per sample than any other single system.
More Metabolites in Less Time
The 4000 Q TRAP® system's high quantitative sensitivity and highly specific scan functions enable you to rapidly, identify and characterize even low-abundance metabolites in a single run. And because this system can trigger MS/MS and MS3 from MRM transitions, you can confirm the presence and structure of metabolites at the same time. So what used to take you multiple analyses on multiple MS platforms, now only takes a single LC/MS/MS run on a single system.
Automated PTM Discovery in a Single Run
Automating PTM analysis is easy using this system's Information Dependent Acquisition (IDA) software, which links highly specific PI and NL scan functions to identify PTMs with high-sensitivity MS/MS scans to determine the modification sites. With the addition of our advanced application software, you have everything you need for automated PTM discovery from a single LC/MS/MS run.
Validate Discovered Protein Biomarkers
Unique MIDASTM workflow enables high throughput validation and quantitation of protein biomarkers discovered through genomic or proteomic experiements.
|TurboIonSpray® probe flow rate range || 5 to 3000 μL/min |
|APCI probe flow rate range || 200 to 3000 μL/min |
|Performance || |
|Dynamic Range || 1 cps to 4 x 106cps (pulse counting) |
|Scan speed || User-settable scan speeds of 250 amu/sec, 1000 amu/sec and 4,000 amu/sec in linear ion trap mode. Maximum of 2,400 amu/sec in RF/DC mode |
|NanoSpray™ source with the MicroIonSpray® head flow rate range || 0.05 to 2.0 μL/min |
|Mass Range || m/z of 5 to 2800 in Q1/Q3 RF/DC mode, m/z of 50 to 2800 in linear ion trap mode |
|NanoSpray™ source using the continuous NanoSpray™ head flow rate || 0.05 to 0.5 μL/min |
|NanoSpray™ source with discrete NanoSpray™ head flow rate || 0.02 to 0.05 μl/min |
|Mass Stability || At vacuum and electronics equilibrium, 0.1 amu over 9 hrs at m/z 2010 with normal operating temperature |
|Mass Resolution || >3000 at m/z 609 at 250 amu/sec |
|Physical Specifications |
|Width || 130 cm (53 in) |
|Depth || 50 cm (20 in) |
|Weight || 136 kg (299 lb) |
|Height || 52 cm (21.5 in) |
|Computer Requirements (PC) |
|Operating System || Windows 2000 |
|CD-ROM || 40x/10x/40x CDRW |
|Floppy || 1.44 MB |
|Memory || 256 MB |
|CPU || 2.53 GHz |
|Hard Disk || 40 GB |
|Display || 18 inches Viewable Flat Panel Monitor recommended |
|Power Requirements |
|Power Consumption || 2500VA max |
|Voltage || 2 separate 208V minimum to 242 V maximum |
|Frequency Hz || 50/60 Hz |
|Circuit Current || 15A grounded single-phase circuit |