continuation from page 2
, page 1
for the Friday morning session was somewhat hard after such a long
evening but obligatory in order not to miss the interesting lecture
given by Alfredo Sanz-Medel (University of Oviedo, Spain) about the
role of ICP-MS in proteomics. His enthusiastic viewpoint about the
important role of ICP-MS for quantification in proteomics stimulated an
intense discussion.Photo 12: Alfredo Sanz-Medel
was followed by Gerhard A. Wiesmüller (Umweltprobenbank des Bundes,
Münster, Germany) who gave an overview of metal species related to
human health emphasizing that more interdisciplinary research is needed
to enlighten the role of metal species in human metabolism.
following presenters concentrated on the development of new methodology
for speciation analysis in the life sciences. Susanne Bomke (University
of Münster, Germany) presented the interesting approach of using
metallocene reagents for the quantification of proteins. Stefan Pieper
(Humboldt University, Berlin, Germany) continued in a similar direction
by using Isotope-coded affinity tags (ICAT) for the absolute
quantification of proteins and peptides using HPLC/ICP MS.
The coffee-break was used for further discussion and the last possibility to visit the exhibition.
second part of the last session started with a contribution by David
Point (NIST, USA) who discussed the influence of sample preparation of
CRMs for the stability of mercury speciation. Using double spike
species specific isotope dilution he was able to follow species
transformation as well as to achieve accurate results. Frank Hasenäcker
(University Duisburg-Essen, Germany) followed with a feasibility study
demonstrating the online coupling of 2D-HPLC and CN-PAGE for native
separation of metalloproteins with subsequent detection by LA-ICP-MS.
Polatajko (ISAS, Dortmund, Germany) discussed the combined use of PAGE
and Nano ESI-FTICR to study metal binding proteins in plants and M.R.
Fernández de la Campa (Department of Physical and Analytical Chemistry,
Oviedo, Spain) presented an approach of the combined use of HPLC-ICPMS
and ESI-MS to study the possible synergic effect of cadmium and
selenium in induced metallothionein. Finally, Norbert Jakubowski (ISAS,
Dortmund, Germany) discussed the possibility of heteroatom tagging as a
tool for quantitative proteomics.
After this very interesting
session on the possibilities of speciation analysis to contribute to
proteomics Uwe Karst announced the Poster awards. He mentioned that the
jury members had a difficult task, since the quality of the posters was
high in general. Altogether, the poster jury had selected 5 posters.
Photo 13: Dieter Klockow (President of IAEAC) presenting the "Roland Frei Award" to Yvonne Scheller (University of Mainz)
The "Roland Frei Award" sponsored by IAEAC with 700 € was given for the
best poster of a young scientists in both poster sessions. The jury
selected Yvonne Scheller from the Johannes Gutenberg-University
Mainz with her work on the "Detection of Ni-species and Co-species in
single living plant cells with high lateral resolution" as the winner
and Dieter Klockow from IAEAC presented the award.
For the second
poster price of the poster session I sponsored by Perkin-Elmer with 300
€, the jury selected Senchao Lai (Johannes Gutenberg-University, Mainz)
with his contribution on "Iodine speciation in the marine aerosol by
inductively coupled plasma isotope dilution mass spectrometry". Harald
Hagendorfer from the Karl-Franzens-University, Graz was nominated as
the winner of the first price for the same session, sponsored by Thermo
with 500 €. Andrew Barker from PS Analytical congratulated Daniel Pröfrock
from the GKSS Research Centre in Geesthacht with his contribution on
the "Hyphenation of capillary LC with ICP-MS and on-line micro fraction
collection for MALDI-TOF analysis as complementary tools for protein
analysis" as the winner of the second poster price of the second poster
session sponsored by PSA with 300 €. Finally, Helmut Feuerbacher from
AHF Analysentechnik presented the first poster award sponsored by his
company with 500 € to Arunachalam Venkatachalam from ISAS - Institute
for Analytical Sciences, Dortmund, for his contribution on "Multiplexed
probing of cytochromes P450 using laser ablation inductively coupled
plasma mass spectrometry (LA-ICP-MS)".
The workshop was than
closed by the organizers by thanking
especially the members of the local committee behind the scene for
their excellent work and all the participants for their contributions
and lively discussion. Finally, the next meeting in this series was
announced to take place September 15-18 in Mainz, Germany, again with
IAEAC and EVISA as co-organizers together with the University of Mainz.Photo 14: Co-chair Michael Sperling (Director of EVISA) closing the Workshop# Related Information Erwin Rosenberg
, Recent trends and progress in trace-element speciation: A report on the international conference "Progress in Analytical Methodologies for Trace Metal Speciation (TraceSpec 2007)", held in Münster, Germany, 4-7 September
, Trends Anal. Chem. (Pers. Ed.), 26/11 (2007) 1013-1018. doi: 10.1016/j.trac.2007.10.007 Michael Sperling
, TraceSpec 2007 in Münster - Analytik der speziellen Art
, GIT Labor Fachzeitschrift, 51/10 (2007) 788-789 (in German).