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Experts in Speciation Analysis came together in Münster, September 4-7, 2007: Part 3

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Attendance for the Friday morning session was somewhat hard after such a long evening but obligatory in order not to miss the interesting lecture given by Alfredo Sanz-Medel (University of Oviedo, Spain) about the role of ICP-MS in proteomics. His enthusiastic viewpoint about the important role of ICP-MS for quantification in proteomics stimulated an intense discussion.

Photo 12: Alfredo Sanz-Medel

He was followed by Gerhard A. Wiesmüller (Umweltprobenbank des Bundes, Münster, Germany) who gave an overview of metal species related to human health emphasizing that more interdisciplinary research is needed to enlighten the role of metal species in human metabolism.

The following presenters concentrated on the development of new methodology for speciation analysis in the life sciences. Susanne Bomke (University of Münster, Germany) presented the interesting approach of using metallocene reagents for the quantification of proteins. Stefan Pieper (Humboldt University, Berlin, Germany) continued in a similar direction by using Isotope-coded affinity tags (ICAT) for the absolute quantification of proteins and peptides using HPLC/ICP MS.

The coffee-break was used for further discussion and the last possibility to visit the exhibition. 
The second part of the last session started with a contribution by David Point (NIST, USA) who discussed the influence of sample preparation of CRMs for the stability of mercury speciation. Using double spike species specific isotope dilution he was able to follow species transformation as well as to achieve accurate results. Frank Hasenäcker (University Duisburg-Essen, Germany) followed with a feasibility study demonstrating the online coupling of 2D-HPLC and CN-PAGE for native separation of metalloproteins with subsequent detection by LA-ICP-MS.

Aleksandra Polatajko (ISAS, Dortmund, Germany) discussed the combined use of PAGE and Nano ESI-FTICR to study metal binding proteins in plants and M.R. Fernández de la Campa (Department of Physical and Analytical Chemistry, Oviedo, Spain) presented an approach of the combined use of HPLC-ICPMS and ESI-MS to study the possible synergic effect of cadmium and selenium in induced metallothionein. Finally, Norbert Jakubowski (ISAS, Dortmund, Germany) discussed the possibility of heteroatom tagging as a tool for quantitative proteomics.

After this very interesting session on the possibilities of speciation analysis to contribute to proteomics Uwe Karst announced the Poster awards. He mentioned that the jury members had a difficult task, since the quality of the posters was high in general. Altogether, the poster jury had selected 5 posters.

Photo 13: Dieter Klockow (President of IAEAC) presenting the "Roland Frei Award" to Yvonne Scheller (University of Mainz)

The "Roland Frei Award" sponsored by IAEAC with 700 € was given for the best poster of a young scientists in both poster sessions. The jury selected  Yvonne Scheller from the Johannes Gutenberg-University Mainz with her work on the "Detection of Ni-species and Co-species in single living plant cells with high lateral resolution" as the winner and Dieter Klockow from IAEAC presented the award.

For the second poster price of the poster session I sponsored by Perkin-Elmer with 300 €, the jury selected Senchao Lai (Johannes Gutenberg-University, Mainz) with his contribution on "Iodine speciation in the marine aerosol by inductively coupled plasma isotope dilution mass spectrometry". Harald Hagendorfer from the Karl-Franzens-University, Graz was nominated as the winner of the first price for the same session, sponsored by Thermo with 500 €. Andrew Barker from PS Analytical congratulated Daniel Pröfrock from the GKSS Research Centre in Geesthacht with his contribution on the "Hyphenation of capillary LC with ICP-MS and on-line micro fraction collection for MALDI-TOF analysis as complementary tools for protein analysis" as the winner of the second poster price of the second poster session sponsored by PSA with 300 €. Finally, Helmut Feuerbacher from AHF Analysentechnik presented the first poster award sponsored by his company with 500 € to Arunachalam Venkatachalam from ISAS - Institute for Analytical Sciences, Dortmund, for his contribution on "Multiplexed probing of cytochromes P450 using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)".

The workshop was than closed by the organizers by thanking especially the members of the local committee behind the scene for their excellent work and all the participants for their contributions and lively discussion. Finally, the next meeting in this series was announced to take place September 15-18 in Mainz, Germany, again with IAEAC and EVISA as co-organizers together with the University of Mainz.

Photo 14: Co-chair Michael Sperling (Director of EVISA) closing the Workshop

# Related Information

Erwin Rosenberg, Recent trends and progress in trace-element speciation: A report on the international conference "Progress in Analytical Methodologies for Trace Metal Speciation (TraceSpec 2007)", held in Münster, Germany, 4-7 September, Trends Anal. Chem. (Pers. Ed.), 26/11 (2007) 1013-1018. doi: 10.1016/j.trac.2007.10.007

Michael Sperling, TraceSpec 2007 in Münster - Analytik der speziellen Art, GIT Labor Fachzeitschrift, 51/10 (2007) 788-789 (in German).

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