Immobilized Liquid Extraction Well Plates for the extraction of organic compounds from aqueous matrices are currently being developed. Parallel sample processing will allow an analyst to extract 96 samples simultaneously with minimal labor in one hour or less. The bottom of each well of the plate is coated with a thin layer of polymer that acts as the extraction medium. Analytes are allowed to partition to equilibrium from a sample by directly exposing the sample to the extracting polymer. Agitation may be provided by a sonicator tray to accelerate partitioning by disrupting a boundary layer which forms at the sample-polymer interface. Analytes that partition into the polymer are then back-extracted into a small amount of solvent, at which point the extraction is complete.
ILE Well Plates allow an analyst to perform extractions both manually and through the use of automated liquid handling systems. Unlike SPE well plates, ILE plates require no vacuum or pressure system, no sorbent conditioning and no collection plate. ILE well plates do not clog, which means no more dry wells. Additionally, Protein precipitation is not required, and derivatizing reagents do not affect the polymer, so back extraction and derivatization can be performed simultaneously.