JRC: STANDARD OPERATING PROCEDURE: DETERMINATION OF METHYLMERCURY IN SEAFOOD BY ELEMENTAL MERCURY ANALIZER
Under request by Directorate General for Health and Consumers (DG SANCO) a collaborative trial, IMEP-115, was conducted in accordance with in ternational protocols by the European Union Reference Laboratory for Heavy Metals in Feed and Food (EURL-HM), to determine the performance char acteristics of an analytical method for the determination of methylmercury in seafood. IMEP-115 was organised in support to th e Commission Regulations 1881/2006 and 882/2004. The method is based on a double liquid-liquid extracti on, first with an organic solvent and then with a cysteine solution. The fi nal quantification is done with a direct mercury analyzer. The repeatability relative standard deviation (RSD r ) ranged from 3.9 to 12.3 % while the reproducibility relative standard deviation (RSD R ) ranged from 8.4 to 24.8 %. The method demonstrates to have acceptable prec ision for all test materials, thus it fit for its intended analytical purpose. Detailed information on the organisation and outcome of the collaborative trial, IMEP- 115, can be found in the report EUR 25830 EN 2013 .