US FDA: High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometric Determination of Four Arsenic Species in Fruit Juice
Version 1.0 (July 2013)
Authors: Sean D. Conklin
This method describes a procedure for using hi gh performance liquid chromatography (HPLC) in combination with inductively coupled plasma-mass spectrometry (ICP-MS) to determine inorganic arsenic (iAs, the sum of arsenite, As(II I) and arsenate, As(V)) in clear (free of solids) fruit juice and fruit juice concentrates. Dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA) are also determined w ith this method. A solution co ntaining arsenobetaine (AsB) and As(III) is analyzed to demonstrate adequate separation between unretained arsenic-containing species and As(III). Other matrices may be analyzed by this procedure if performance is verified in the matrix of interest and at the concentration of interest. This method should be used by analysts experien ced in the use of HPLC and ICP-MS, including the identification of chromatographic and matrix interferences and procedures for their correction and should be used only by personnel thoroughly trained in the handling and analysis of samples for determination of trace elements in food products.