Do you need to detect low-level contaminants? Does your method require detection at different wavelengths? Do you need information about coeluting compounds? Then the UVD 170U multichannel UV-Vis detector is the right choice. High sensitivity for all four channels is assured using discrete, large-area photodiode arrays (PDA). As no moving parts are necessary to simultaneously acquire up to four wavelengths, the UVD 170U achieves a noise level of <±5 µAU (dry cell, 254 nm)—a level normally achieved only by single wavelength detectors. This level enables lower limits of detection, including even trace-level impurities. Coeluting impurities can be detected by dividing the absorbance signal of a peak at two selected wavelengths, calculating a signal ratio. If a coeluting impurity is present, this causes the signal ratio to change across the peak. Adapt the UVD 170U to microbore or preparative applications using the available optional flow cells.
Reliable Quantification The UVD 170U is based on PDA technology and an advanced optical design. Unlike traditional PDA optics, however, the UVD 170U uses large surface-area discrete diodes, yielding significantly enhanced sensitivity. This enhanced sensitivity results in a noise level normally only achieved by single wavelength detectors, reaching below ±5 µAU (dry cell, 254 nm), enabling lower limits of detection that includes even trace-level impurities.
Maximum Sensitivity with Multiwavelength Detection Many standard methods require detection of analytes at several different wavelengths. Most UV-Vis detectors can acquire 2 or more wavelength signals, but this generally results in a loss in sensitivity, due to their moving parts. Using PDA technology, the UVD 170U achieves multiwavelength detection without moving parts, recording up to four truly synchronous wavelengths, all at the same high sensitivity. This high sensitivity allows even complex mixtures to be analyzed in a single run. Each analyte is detected at its optimum wavelength, with maximum sensitivity. The Chromeleon® report generator allows the display of amounts determined from several channels in a single report table, avoiding confusing multipage reports. An additional advantage of the PDA design is its superior robustness and reliability compared to monochromator-based instruments with moving optical components.
Information on Coeluting Compounds Chomeleon divides the absorbance signal of a peak at two selected wavelengths to calculate a signal ratio. A coeluting impurity will cause the signal ratio to change across the peak, due to different absorption characteristics of the main compound and impurity. In this way, signal ratio plots can serve as a simple tool for peak purity analysis. These plots are calculated for any combination of the four wavelength channels of the UVD 170U and can be displayed in the chromatogram.
Adaptability and Application Flexibility The UVD 170U provides the flexibility you need to run all your HPLC applications under optimal conditions. A fused, silica, nano flow cell with 395-nL cell volume and 8-mm path length is ideally suited for applications using 1- to 2-mm i.d. columns, (e.g., for microbore LC/MS). For applications using 2- to 3-mm i.d. columns, a micro flow cell with 2.5 µL cell volume and 9-mm path length is recommended. In semipreparative applications, the path length of the flow cell is usually reduced to allow for high sample concentrations. For this purpose, a semipreparative flow cell with 6-µL cell volume and 2-mm path length is available.
Easy Setup, Maintenance, and Validation USB and Ethernet interfaces make the UVD 170U simple to install and compatible with state-of-the-art communication interfaces. Control through USB provides for plug-and-play installation within minutes. An integrated USB hub allows the connection of up to three additional USB devices directly to the detector. Fast access to the lamp and flow cell minimize instrument downtime during maintenance: the UVD is up running again within minutes. Summit System Wellness features like minimum lamp intensity, lamp age, and lamp ignitions make sure you always use the UVD under optimal conditions. Wavelength verification through a built-in holmium oxide filter ensures correct calibration of the UVD 170U to within ±0.75 mm. Powerful and easy to use AutoQ TM validation routines allow for automated Installation Qualification (IQ), Operational Qualification (OQ), and Performance Qualification (PQ) tests. OQ and PQ provide verification of wavelength accuracy, noise, drift, linearity, and lamp intensity.
Secure Investment: Upgradeable to a Photodiode Array Detector As the UVD 170U is based on photodiode array (PDA) technology, it possesses the necessary foundation for a full-featured PDA detector. The unit can be field-upgraded at any time to the full PDA version, the UVD 340U, at a fraction of the cost of a new instrument. Thus, you can purchase the unit you need today, secure in the knowledge that it can adapt to your future analysis needs.
<±0.5 × 10–5AU, after 3-h warmup at 254 nm, step 1 s, bandwidth 8 nm, dry flow cell
<5 × 10–4AU/h, after 3-h warmup at 254 nm, dry flow cell
±0.75 nm (UV range) ±1.5 nm (VIS range)
1.9 nm (UV range) 3.3 nm (VIS range)
<1% deviation at 1.5 AU
0.1–4.0 s, programmable
Power up wavelength verification
Automatic through internal holmium oxide filter
Automatic or on demand
Single-beam principle with simultaneous measurement across the photodiode array
Photodiode array bench without moving parts
Through USB or Ethernet (RJ-45 connection)
4 detection channels, digitally transmitted
2 digital Inputs/Outputs; can be used as relay outputs, default open, or as TTL compatible inputs
Additional USB ports
Integrated USB hub with three type A USB ports
Flow cells (volume, path length, pressure limit)
Standard analytical cell
10 µL, 9 mm, 10 MPa (1450 psi)
Biocompatible cell (optional)
10 µL, 9 mm, 10 MPa (1450 psi)
2.5 µL, 9 mm, 10 MPa (1450 psi)
Fused silica nanocell (optional)
395 nL, 8 mm, 40 Mpa (5800 psi)
Semipreparative cell (optional)
6 µL, 2 mm, 10 Mpa (1450 psi)
115/230 V ± 10%, 50/60 Hz, 50 VA
Temperature: 10–35 °C (50–95 °F) Air humidity: 40–85% relative humidity, noncondensing Overvoltage category: II Pollution degree: 2