Applied Biosystems - 4700 Proteomics Analyzer
| Year of introduction
Mass spectrometer: MALDI
The Applied Biosystems 4700 Proteomics Analyzer with TOF/TOF™ optics is a MALDI tandem time-of-flight mass spectrometer that provides a new level of productivity in high-resolution proteome analysis and complex systems biology.
- Fast and flexible mass spectrometry and automated results dependent analysis using RDA™ Software maximizes experimental efficiency.
- High-sensitivity instrument performance with wide dynamic range allows you to target low-abundance constituents of complex protein mixtures.
- Superior peak detection, reproducible spectral quality and accurate mass provide reliable protein identification and enhanced characterization.
- Information-rich fragmentation patterns unavailable on other instrument platforms for carbohydrate, lipid, and other biological component analysis.
- Ultra-Wide Mass Range of TOF/TOF™ optics enables you to analyze large proteins and peptides.
True Innovation in Mass Spectrometry Technology For Research Use Only. Not for use in diagnostic procedures.
As the first commercial instrument to deliver the high sensitivity of MALDI coupled with true MS/MS, the 4700 Proteomics Analyzer with TOF/TOF™ optics yields reproducible, high-quality spectra for confident protein identification and quantification. This mass spectrometer is the only TOF/TOF system that can perform rapid MS and MS/MS, using automated acquisition that maximizes the depth of protein coverage and dynamic range.
Reliable Results for Your Biological Samples
Data from the 4700 Proteomics Analyzer provides the reproducible spectral quality and accurate mass you need to confidently target and identify proteins of interest by gel-based and LC MALDI workflows. To help you understand the underlying biology of disease, the 4700 Proteomics Analyzer has flexible and easy-to-use acquisition and analysis tools to automate data collection and identification based on the experiment that best interrogates your biological samples.
Computer Requirements (PC)
System Requirements Windows 2000
Operating System Microsoft® Office XP Professional, Integrated Oracle® Database
Hard Disk Two 80-GB HDD
CPU Dual 850 MHz CPU
Memory 512-MB RAM
Floppy System Software: AB 4700 Explorer™ Software, AB Data Explorer™
CD-ROM CD ROM. Rewritable
Display 19" Flat Panel Monitor
Throughput Up to 36,000 PMF's per day with optional automation package
Mass Accuracy MS/MS Mode: Angiotensin fragments 354 - 1183 +/- 50 ppm
Mass Resolution GluFib fragments; 175 > 2000 res., 684 & 1056 > 3500 res., 1441 > 5000 res.
Sensitivity Refl. Mode: 5 fm Neurotenisin m/z 1672 50:1 s/n : MS/MS Mode: Angiotensin fragment 926 10:1 s/n
Laser Solid state, Integrated
Laser rep rate Up to 200Hz
Data acquisition Up to 200Hz
Width 93 9/32 inches (237.0 cm)
Weight ~1800 pounds (816 kg)
Depth 33 1/4 inches (84.5 cm)
Height 50 3/16 inches (127.5 cm)
Voltage 100, 120, 220, 240 Selectable
Power Consumption < 1000W
Power Dissipated (W) 2300 BTU/Hr dissipated into the room
Circuit Current Requires 15A service, draws 3.6 - 8.8 pending input voltage
Frequency Hz 50-60Hz
System Setup Specifications
Temperature Accuracy Requires 20 - 25 deg. C, constant within < 2 deg. C variation over 24 hours
Standard Software Functions Tuning, Calibration, Manual and Automatic Data Acquisition, Diagnostics
Gas Supplies 3 connections for Collision Gases, N2 & 2 optional
Installation Requirements See Site Preparation Guide Document
Mass accuracy, reflector, internal Using Angio 1296 & ACTH 2465 as calibrant, peaks measure GluFib 1570 & ACTH 2093 (as unknowns); Internal Calibration +/- 5 ppm
Mass accuracy, reflector, external Using Angio 1296 & ACTH 2465 as calibrant, peaks measure GluFib 1570 & ACTH 2093 (as unknowns); Ext. Calibration +/- 25 ppm
Resolution Reflector Angio 1296, GluFib 1570, ACTH 2093, ACTH 2465, ACTH 3657; all >12,000 res. @ 200:1 s/n (measured @ 2465 peak)
Resolution Linear GluFib 1570 >3000 res. @ 200:1 s/n